Catalog No: OKEH04825
Size:96T
Price: $525.00
SKU
OKEH04825
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology MAGEA1 ELISA Kit (Human) (OKEH04825) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAGEA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAGEA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAGEA1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
Sensitivity | 0.326 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Melanoma-associated antigen 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | MAGEA1 |
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Gene Full Name | MAGE family member A1 |
Alias Symbols | antigen MZ2-E;cancer/testis antigen 1.1;cancer/testis antigen family 1, member 1;CT1.1;MAGE1;MAGE-1 antigen;melanoma antigen family A 1;melanoma antigen family A, 1 (directs expression of antigen MZ2-E);melanoma antigen family A1;melanoma antigen MAGE-1;melanoma-associated antigen 1;melanoma-associated antigen MZ2-E. |
NCBI Gene Id | 4100 |
Protein Name | P43355 |
Description of Target | Melanoma-associated antigen 1 |
Uniprot ID | https://www.uniprot.org/uniprot/P43355 |
Protein Accession # | NP_004979.3 |
Nucleotide Accession # | NM_004988.4 |
Protein Size (# AA) | 309 |
Molecular Weight | 34 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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