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Catalog No: OKEH04825
Size:96T
Price: $525.00
SKU
OKEH04825
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology MAGEA1 ELISA Kit (Human) (OKEH04825) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAGEA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAGEA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAGEA1 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
MAGEA1 Microplate96 Wells (12 x 8 Well Strips)
MAGEA1 Standard2
Sample Diluent1 x 20 mL
MAGEA1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.326 ng/mL
SpecificityNatural and recombinant Human Melanoma-associated antigen 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMAGEA1
Gene Full NameMAGE family member A1
Alias Symbolsantigen MZ2-E;cancer/testis antigen 1.1;cancer/testis antigen family 1, member 1;CT1.1;MAGE1;MAGE-1 antigen;melanoma antigen family A 1;melanoma antigen family A, 1 (directs expression of antigen MZ2-E);melanoma antigen family A1;melanoma antigen MAGE-1;melanoma-associated antigen 1;melanoma-associated antigen MZ2-E.
NCBI Gene Id4100
Protein NameP43355
Description of TargetMelanoma-associated antigen 1
Uniprot IDhttps://www.uniprot.org/uniprot/P43355
Protein Accession #NP_004979.3
Nucleotide Accession #NM_004988.4
Protein Size (# AA)309
Molecular Weight34 kDa
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