M-CSF ELISA Kit (Human) (OKBB00211)

Catalog No: OKBB00211

Size:96 Tests

Human CSF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli, E33-S190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human M-CSF amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	546	1011	2456	623	1528	2629
  St. Dev.	36.6	44.5	125.3	43.61	85.57	152.5
  %CV	6.70	4.40	5.10	7	5.60	5.80

• ELISA Kit Component:
  • Lyophilized recombinant human IL-12(p40) standard: 10 ng/tube x 2
  • Anti-human IL-12(p40) Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL-12(p40) antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 62.5pg/ml-4000pg/ml
  Principle: Aviva’s human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human M-CSF specific polyclonal antibody was pre-coated onto 96-well plates. The human specific detection polyclonal antibody was biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human M-CSF amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine
• Reference: 1. Pollard, J. W.; Bartocci, A.; Arceci, R.; Orlofsky, A.; Ladner, M. B.; Stanley, E. R. : Apparent role of the macrophage
  growth factor, CSF-1, in placental development. Nature 330: 484-486, 1987.
  2. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. : Human CSF-1: gene
  structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
  3. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. : Human CSF-1: gene
  structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
• Specificity: Natural and recombinant human M-CSF
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: E33-S190
• Description: For quantitative detection of human M-CSF in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

Target Info

• Gene Symbol: CSF1
• Gene Full Name: colony stimulating factor 1
• Alias Symbols: Colony stimulating factor 1, CSF-1, CSF1, Lanimostim, MCSF, P09603, CSF 1, M CSF, M-CSF
• NCBI Gene Id: 1435
• Protein Name: Macrophage colony-stimulating factor 1
• Description of Target: SF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
• Uniprot ID: P09603
• Protein Accession #: NP_000748.3
• Nucleotide Accession #: NM_000757.5