LYZ ELISA Kit (Chicken) (OKEH03977)

Catalog No: OKEH03977

Size:96T

Chicken LYZ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology LYZ ELISA Kit (Chicken) (OKEH03977) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LYZ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LYZ is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LYZ captured in well.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Component:
  • LYZ Microplate: 96 Wells (12 x 8 Well strips)
  • LYZ Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated LYZ Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 1.39 ng/mL
• Specificity: Natural and recombinant Chicken Lysozyme C
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LYZ
• Gene Full Name: lysozyme (renal amyloidosis)
• Alias Symbols: 1,4-beta-N-acetylmuramidase C;4-beta-N-acetylmuramidase C;allergen Gal d IV;dystrophin;egg white lysozyme;lysozyme C;LYZC.
• NCBI Gene Id: 396218
• Protein Name: P00698
• Description of Target: Lysozyme C
• Uniprot ID: https://www.uniprot.org/uniprot/P00698
• Protein Accession #: NP_990612.1
• Nucleotide Accession #: NM_205281.1
• Protein Size (# AA): 147
• Molecular Weight: 147 kDa