LY96 ELISA Kit (Human) (OKCD00545)

Catalog No: OKCD00545 (Formerly GWB-KBBPN6)

Size:96WELLS

Human LY96 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 96 (LY96). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 96 (LY96). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 96 (LY96), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphocyte Antigen 96 (LY96) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 96 (LY96) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Antigen 96 (LY96) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LY96 Microplate: 96 Wells (12 x 8 Well Strips)
  • LY96 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated LY96 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 96 (LY96). No significant cross-reactivity or interference between Lymphocyte Antigen 96 (LY96) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LY96
• Gene Full Name: lymphocyte antigen 96
• Alias Symbols: ESOP-1;ly-96;lymphocyte antigen 96;MD2;MD-2;myeloid differentiation protein-2;protein MD-2.
• NCBI Gene Id: 23643
• Protein Name: Lymphocyte antigen 96
• Description of Target: Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).
• Uniprot ID: Q9Y6Y9
• Protein Accession #: NP_001182726
• Nucleotide Accession #: NM_001195797.1
• Protein Size (# AA): 160