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Catalog No: OKCD00285 (Formerly GWB-KBBYF3)
Size:96WELLS
Price: $650.00
SKU
OKCD00285
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Datasheets/ManualsPrintable datasheet for LY75 ELISA Kit (Mouse) (OKCD00285)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Ly75 Microplate96 Wells (12 x 8 Well Strips)
Ly75 Lyophilized Standard2 x 10 ng
100X Biotinylated Ly75 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell Lysates and other biological fluids.
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 75 (LY75). No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLy75
Gene Full Namelymphocyte antigen 75
Alias SymbolsCD205;DEC205;DEC-205;ly-75;lymphocyte antigen 75.
NCBI Gene Id17076
Protein NameLymphocyte antigen 75
Description of TargetActs as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).
Uniprot IDQ60767
Protein Accession #NP_038853
Nucleotide Accession #NM_013825.3
Protein Size (# AA)1723
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