LXR-A Antibody (OAPB01031)

Catalog No: OAPB01031

Size:100UG

Western blot analysis of LXR-A in rat liver tissue lysate with LXR-A antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemistry of LXR-A in rat liver tissue with LXR-A antibody at 5 μg/mL.

Immunofluorescence of LXR-A in rat liver tissue with LXR-A antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. LXR-A Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: LXR-A antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: LXR-A antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus human LXR-A. The immunogen is located within amino acids 50 - 100 of LXR-A.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-LXR-A antibody is Catalog # OEPB03143
• Application Info: LXR-A antibody can be used for detection of LXR-A by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Willy PJ, Umesono K, Ong ES, et al. LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev.1995; 9:1033-45.;Edwards PA, Kennedy MA, and Mak PA. LXRs; Oxysterol-activated nuclear receptors that regulate genes controlling lipid homeostasis. Vasc. Pharmacol.2002; 38:249-56.;Bensinger SJ and Tontonoz P. Integration of metabolism and inflammation by lipid-activated nuclear receptors. Nature2008; 454:470-7.;Villablanca EJ, Raccosta L, Zhou D, et al. Tumor-mediated liver X receptor-a activation inhibits CC chemokine reeptor-7 expression on dendritic cells and dampens antitumor responses. Nature Med.2010; 16:98-106.

Target Info

• Gene Symbol: NR1H3
• Gene Full Name: nuclear receptor subfamily 1 group H member 3
• Alias Symbols: liver X nuclear receptor alpha variant 1;Liver X receptor alpha;LXR-a;LXRA;Nuclear receptor subfamily 1 group H member 3;oxysterols receptor LXR-alpha;RLD-1.
• NCBI Gene Id: 10062
• Protein Name: Oxysterols receptor LXR-alpha|oxysterols receptor LXR-alpha isoform 1 [Homo sapiens]
• Description of Target: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).
• Uniprot ID: Q13133
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_005684
• Molecular Weight: Predicted: 44, 49 kDa Observed: 46 kDa