Luteinizing Hormone ELISA Kit (Mouse) : 96 Wells (OKCA00156)

Catalog No: OKCA00156

Size:96 Wells

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	87%	85-92%
  Serum (n=4)	1:2	92%	87-99%
  Serum (n=4)	1:4	95%	88-101%
  Serum (n=4)	1:8	96%	92-105%

• ELISA Kit Principle: Aviva Systems Biology Luteinizing Hormone ELISA Kit (Mouse) (OKCA00156) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A mouse monoclonal antibody specific for Mouse Luteinizing Hormone has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Mouse Luteinizing Hormone is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse Luteinizing Hormone captured in well.
• ELISA Kit Range: 2 mIU/mL - 75 mIU/mL
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (mIU/mL)	20.348	18.906	19.157	20.022	18.69	19.733
  SD	0.041	0.038	0.04	0.057	0.063	0.063
  CV%	6.077	5.984	6.231	8.557	10.011	9.574

• ELISA Kit Component:
  • Anti-Luteinizing Hormone Microplate: 96 Well (12 x 8 Well Strips)
  • Luteinizing Hormone Standard (Lyophilized): 6
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: 0.5 mIU/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse
  LH. No significant cross-reactivity or interference between mouse LH and
  analogues was observed.
• Assay Info: Assay Methodology: Qualitative Sandwich Immunoassay

Target Info

• Gene Symbol: Luteinizing Hormone