Catalog No: OKCD01174 (Formerly GWB-KBBKR3)
Size:96WELLS
Price: $650.00
SKU
OKCD01174
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Datasheets/ManualsPrintable datasheet for LTF ELISA Kit (Rat) (OKCD01174)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-97%92-99%87-95%82-101%
EDTA plasma(n=5)97-104%93-101%83-97%80-94%
heparin plasma(n=5)79-90%80-89%94-105%83-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactoferrin (LTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lactoferrin (LTF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range6.25-400ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-104101
EDTA plasma(n=5)78-9881
heparin plasma(n=5)78-8882
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Ltf Microplate96 Wells (12 x 8 Well Strips)
Ltf Lyophilized Standard2 x 400 ng
100X Biotinylated Ltf Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 2.84 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF). No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLtf
Gene Full Namelactotransferrin
Alias Symbolslactoferrin;lactotransferrin.
NCBI Gene Id301034
Protein NameLactotransferrin (Predicted)
Description of TargetLactotransferrin (Predicted)
Uniprot IDD3ZAB1
Protein Accession #NP_001100334
Nucleotide Accession #NM_001106864.1
Protein Size (# AA)709
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