LTBR ELISA Kit (Human) (OKCD00802)

Catalog No: OKCD00802 (Formerly GWB-KBBLV6)

Size:96WELLS

Human LTBR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphotoxin Beta Receptor (LTbR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphotoxin Beta Receptor (LTbR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphotoxin Beta Receptor (LTbR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphotoxin Beta Receptor (LTbR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphotoxin Beta Receptor (LTbR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphotoxin Beta Receptor (LTbR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LTBR Microplate: 96 Wells (12 x 8 Well Strips)
  • LTBR Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated LTBR Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphotoxin Beta Receptor (LTbR). No significant cross-reactivity or interference between Lymphotoxin Beta Receptor (LTbR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LTBR
• Gene Full Name: lymphotoxin beta receptor
• Alias Symbols: D12S370;LT-BETA-R;lymphotoxin B receptor;lymphotoxin beta receptor (TNFR superfamily, member 3);Lymphotoxin-beta receptor;TNFCR;TNFR2-RP;TNFR3;TNF-R-III;TNFR-RP;TNFRSF3;tumor necrosis factor C receptor;tumor necrosis factor receptor 2-related protein;tumor necrosis factor receptor superfamily member 3;tumor necrosis factor receptor type III.
• NCBI Gene Id: 4055
• Protein Name: Tumor necrosis factor receptor superfamily member 3
• Description of Target: Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
• Uniprot ID: P36941
• Protein Accession #: NP_001257916
• Nucleotide Accession #: NM_001270987.1
• Protein Size (# AA): 435