LRRC3C ELISA Kit (Human) (OKCD00648)

Catalog No: OKCD00648 (Formerly GWB-KBBWC8)

Size:96WELLS

Human LRRC3C ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat Containing Protein 3C (LRRC3C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Rich Repeat Containing Protein 3C (LRRC3C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LRRC3C Microplate: 96 Wells (12 x 8 Well Strips)
  • LRRC3C Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated LRRC3C Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.066 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing Protein 3C (LRRC3C). No significant cross-reactivity or interference between Leucine Rich Repeat Containing Protein 3C (LRRC3C) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: LRRC3C
• Gene Full Name: leucine rich repeat containing 3C
• Alias Symbols: leucine-rich repeat-containing protein 3C;putative LRRC3-like protein ENSP00000367157.
• NCBI Gene Id: 100505591
• Protein Name: Leucine-rich repeat-containing protein 3C
• Description of Target: Leucine-rich repeat-containing protein 3C
• Uniprot ID: A6NJW4
• Protein Accession #: NP_001182474
• Nucleotide Accession #: NM_001195545.1
• Protein Size (# AA): 275