Catalog No: OKEH03236
Size:96T
Price: $525.00
SKU
OKEH03236
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LRPPRC ELISA Kit (Human) (OKEH03236) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LRPPRC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for LRPPRC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LRPPRC captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.2% (n = 20)
Mean Inter-assay CV%: <= 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-LRPPRC Microplate96 Wells (12 x 8 Well strips)
LRPPRC Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated LRPPRC Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.03 pg/mL
SpecificityNatural and recombinant Human Leucine-rich PPR motif-containing protein, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLRPPRC
Gene Full Nameleucine rich pentatricopeptide repeat containing
Alias Symbols130 kDa leucine-rich protein;CLONE-23970;GP130;leucine-rich PPR motif-containing protein, mitochondrial;leucine-rich PPR-motif containing;LRP 130;LRP130;LSFC;MC4DN5;mitochondrial leucine-rich PPR motif-containing protein.
NCBI Gene Id10128
Protein NameP42704
Description of TargetLeucine-rich PPR motif-containing protein, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P42704
Protein Accession #NP_573566.2
Nucleotide Accession #NM_133259.3
Protein Size (# AA)1394
Molecular Weight158 kDa
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