LRG1 High Sensitivity ELISA Kit (Human) (OKCD01356)

Catalog No: OKCD01356

Size:96WELLS

Human LRG1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-101%	80-101%	91-101%	97-105%
  EDTA Plasma (n=5)	78-98%	86-96%	80-94%	93-101%
  Heparin Plasma (n=5)	90-97%	96-105%	89-96%	96-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	83-97	90
  Heparin Plasma (n=5)	86-96	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LRG1 Microplate: 96 Wells (12 x 8 Well Strips)
  • LRG1 Lyophilized Standard: 2
  • 100X Biotinylated LRG1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Alpha-2-Glycoprotein 1 (LRG1). No significant cross-reactivity or interference between Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LRG1
• Gene Full Name: leucine rich alpha-2-glycoprotein 1
• Alias Symbols: 1300008B03Rik;2310031E04Rik;HMFT1766;leucine rich alpha 2 glycoprotein;leucine-rich alpha-2-glycoprotein;LRG.
• NCBI Gene Id: 116844
• Protein Name: Leucine-rich alpha-2-glycoprotein
• Description of Target: Leucine-rich alpha-2-glycoprotein
• Uniprot ID: P02750
• Protein Accession #: NP_443204.1
• Nucleotide Accession #: NM_052972.2