LPL ELISA Kit (Sheep) (OKCA01960)

Catalog No: OKCA01960

Size:96 Wells

Sheep LPL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution

  Mean Recovery (%)

  Range (%)

• ELISA Kit Principle: Aviva Systems Biology LPL ELISA Kit (Sheep) (OKCA01960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPL has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LPL captured in the well.
• ELISA Kit Range: 7.8 - 500 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	102	93-106
  EDTA Plasma (n=4)	103	94-107

• ELISA Kit Component:
  • LPL Microplate: 96 Wells (12 x 8 Well strips)
  • LPL Lyophilized Standard: 2 x 500 ng
  • 100X Biotinylated LPL Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.95 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: LPL
• Gene Full Name: Lipoprotein lipase
• Alias Symbols: HDLCQ11, LIPD
• NCBI Gene Id: 443408
• Protein Name: Lipoprotein lipase
• Description of Target: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
• Uniprot ID: Q29524
• Protein Accession #: NP_001009394.1
• Nucleotide Accession #: NM_001009394.1