Catalog No: OKCD02703
Size:96WELLS
Price: $700.00
SKU
OKCD02703
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Datasheets/ManualsPrintable datasheet for LPCAT3 ELISA Kit (Mouse) (OKCD02703)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-104%84-98%82-89%84-95%
EDTA Plasma (n=5)93-101%93-101%90-102%89-96%
Heparin Plasma (n=5)86-97%92-101%96-104%79-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 3 (LPCAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-10496
EDTA Plasma (n=5)91-10399
Heparin Plasma(n=5)78-9487
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LPCAT3 Microplate96 Wells (12 x 8 Well strips)
LPCAT3 Lyophilized Standard2
100X Biotinylated LPCAT3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLpcat3
Gene Full Namelysophosphatidylcholine acyltransferase 3
Alias Symbols1-acylglycerophosphocholine O-acyltransferase;1-acylglycerophosphoethanolamine O-acyltransferase;1-acylglycerophosphoserine O-acyltransferase;C3f;Grcc3;Grcc3f;LPC acyltransferase 3;Lpcat;Lpeat;Lplat5;Lpsat;lyso-PC acyltransferase 3;lyso-PE acyltransferase;Lysophosphatidylcholine acyltransferase;Lysophosphatidylcholine acyltransferase 3;lysophosphatidylethanolamine acyltransferase;lysophosphatidylserine acyltransferase;lysophospholipid acyltransferase 5;lyso-PS acyltransferase;Mboa;Mboat5;Membrane-bound O-acyltransferase domain-containing protein 5;Moact5;Oac;Oact5;O-acyltransferase domain-containing protein 5;PTG.
NCBI Gene Id14792
Protein NameLysophospholipid acyltransferase 5
Description of TargetAcyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). To a lesser extent, also catalyzes the acylation of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity), and the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.
Uniprot IDQ91V01
Protein Accession #NP_660112.1
Nucleotide Accession #NM_145130.2
Protein Size (# AA)487
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