Catalog No: OKCD02703
Size:96WELLS
Price: $700.00
SKU
OKCD02703
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for LPCAT3 ELISA Kit (Mouse) (OKCD02703) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 3 (LPCAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.055 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Lpcat3 |
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Gene Full Name | lysophosphatidylcholine acyltransferase 3 |
Alias Symbols | 1-acylglycerophosphocholine O-acyltransferase;1-acylglycerophosphoethanolamine O-acyltransferase;1-acylglycerophosphoserine O-acyltransferase;C3f;Grcc3;Grcc3f;LPC acyltransferase 3;Lpcat;Lpeat;Lplat5;Lpsat;lyso-PC acyltransferase 3;lyso-PE acyltransferase;Lysophosphatidylcholine acyltransferase;Lysophosphatidylcholine acyltransferase 3;lysophosphatidylethanolamine acyltransferase;lysophosphatidylserine acyltransferase;lysophospholipid acyltransferase 5;lyso-PS acyltransferase;Mboa;Mboat5;Membrane-bound O-acyltransferase domain-containing protein 5;Moact5;Oac;Oact5;O-acyltransferase domain-containing protein 5;PTG. |
NCBI Gene Id | 14792 |
Protein Name | Lysophospholipid acyltransferase 5 |
Description of Target | Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). To a lesser extent, also catalyzes the acylation of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity), and the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. |
Uniprot ID | Q91V01 |
Protein Accession # | NP_660112.1 |
Nucleotide Accession # | NM_145130.2 |
Protein Size (# AA) | 487 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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