Catalog No: OKCD02696
Size:96WELLS
Price: $700.00
SKU
OKCD02696
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Datasheets/ManualsPrintable datasheet for LOX ELISA Kit (Mouse) (OKCD02696)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-96%79-93%92-105%90-101%
EDTA Plasma (n=5)79-88%82-103%78-89%83-93%
Heparin Plasma (n=5)86-104%80-89%87-94%79-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase (LOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase (LOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase (LOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl Oxidase (LOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-10185
EDTA Plasma (n=5)78-9483
Heparin Plasma(n=5)78-10484
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LOX Microplate96 Wells (12 x 8 Well strips)
LOX Lyophilized Standard2
100X Biotinylated LOX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.117 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase (LOX). No significant cross-reactivity or interference between Lysyl Oxidase (LOX) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLox
Gene Full Namelysyl oxidase
Alias SymbolsAI893619;Lysyl oxidase;protein-lysine 6-oxidase;ras excision protein;rrg;TSC-16;TSC-160.
NCBI Gene Id16948
Protein NameProtein-lysine 6-oxidase
Description of TargetResponsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (PubMed:27432961).
Uniprot IDP28301
Protein Accession #NP_001273110.1
Nucleotide Accession #NM_001286181.1
Protein Size (# AA)411
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