LOX-1 ELISA Kit (Mouse) (OKBB00334)

Catalog No: OKBB00334

Size:96 Tests

Mouse OLR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LOX-1 has been precoated onto 96-well plates. Standards(NS0, R60-I363) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOX-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	176	748	1336	224	862	1428
  St. Dev.	7.57	38.9	86.84	12.1	52.6	107.1
  %CV	4.30	5.20	6.5	5.40	6.10	7.5

• ELISA Kit Component:
  • Lyophilized recombinant mouse C-MET standard: 10 ng/tube x 2
  • Anti-mouse C-MET Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-mouse C-MET antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 31.2pg/ml-2000pg/ml;
  Principle: Aviva’s mouse LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse LOX-1 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using.
  Background: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Li X, Bouzyk MM, Wang X (Nov 1998). "Assignment of the human oxidized low-density lipoprotein receptor gene
  (OLR1) to chromosome 12p13.1 => p12.3, and identification of a polymorphic CA-repeat marker in the OLR1 gene".
  Cytogenet Cell Genet 82 (1-2): 34–6. doi:10.1159/000015059. PMID 9763655.
  2. Honjo, M., Sawamura, T., Hinagata, J., Nakamura, K., Sanada, N., Tanihara, H., Honda, Y., Kiryu, J. Expression of
  LOX-1, an oxidized low-density lipoprotein receptor, in choroidal neovascularization. Arch. Ophthal. 122: 1873-1876, 2004.
  [PubMed: 15596594]
• Specificity: Natural and recombinant mouse LOX-1
• Immunogen: Expression system for standard: NS0; Immunogen sequence: R60-I363
• Description: For quantitative detection of mouse LOX-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: OLR1
• Gene Full Name: oxidized low absorbance lipoprotein (lectin-like) receptor 1
• Alias Symbols: C-type lectin domain family 8 member A, CLEC8A, hLOX-1, Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, LOXIN, Olr1, OLR1_HUMAN, Ox-LDL receptor 1, Oxidized low absorbance lipoprotein (lectin like) receptor 1, Oxidized low absorbance lipoprotein receptor 1 soluble form, Oxidized low-absorbance lipoprotein receptor 1, SCARE1, Scavenger receptor class E member 1, SLOX1
• NCBI Gene Id: 108078
• Protein Name: Oxidized low-absorbance lipoprotein receptor 1
• Description of Target: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.
• Uniprot ID: Q9EQ09
• Protein Accession #: NP_619589.2
• Nucleotide Accession #: NM_138648.2