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Catalog No: OKEH06712
Size:96T
Price: $625.00
SKU
OKEH06712
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Lmx1b ELISA Kit (Mouse) (OKEH06712) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lmx1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lmx1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lmx1b captured in the well.
ELISA Kit Range0.625-40ng/ml
ELISA Kit RecoveryMean recovery when spiking into serum = 94%
ELISA Kit ReproducibilityIntra-Assay Reproducibility CV% < 8.3% (n=20)< br>Inter-Assay Reproducibility CV% < 10.4% (n=24)
ELISA Kit Component
ComponentAmount
MSH2 Microplate96 Wells (12 x 8 Well strips)
MSH2 Lyophilized Standard2 x 40 ng
100X Biotinylated MSH2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.625 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLmx1b
Gene Full NameLIM homeobox transcription factor 1 beta
Alias SymbolsGENA 191;Ic;Icst;LIM homeo box transcription factor 1B;LIM homeobox transcription factor 1-beta;LIM/homeobox protein 1.2;LIM/homeobox protein LMX1B;LMX1.;LMX1.1;LMX1.2;LMX-1.2.
NCBI Gene Id16917
Protein NameO88609
Description of TargetLIM homeobox transcription factor 1-beta
Uniprot IDhttps://www.uniprot.org/uniprot/O88609
Protein Accession #NP_034855
Nucleotide Accession #NM_010725
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