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Catalog No: OKCD00469 (Formerly GWB-KBBYX5)
Size:96WELLS
Price: $675.00
SKU
OKCD00469
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Datasheets/ManualsPrintable datasheet for LMNA ELISA Kit (Human) (OKCD00469)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin A/C (LMNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin A/C (LMNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin A/C (LMNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lamin A/C (LMNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LMNA Microplate96 Wells (12 x 8 Well Strips)
LMNA Lyophilized Standard2 x 10 ng
100X Biotinylated LMNA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lamin A/C (LMNA). No significant cross-reactivity or interference between Lamin A/C (LMNA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLMNA
Gene Full Namelamin A/C
Alias Symbols70 kDa lamin;CDCD1;CDDC;CMD1A;CMT2B1;EMD2;epididymis secretory sperm binding protein;FPL;FPLD;FPLD2;HGPS;IDC;lamin;lamin A/C-like 1;LDP1;LFP;LGMD1B;LMN1;LMNC;LMNL1;MADA;mandibuloacral dysplasia type A;prelamin-A/C;PRO1;renal carcinoma antigen NY-REN-32.
NCBI Gene Id4000
Protein NamePrelamin-A/C
Description of TargetLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation (PubMed:10080180, PubMed:22431096, PubMed:10814726, PubMed:11799477, PubMed:18551513). Required for osteoblastogenesis and bone formation (PubMed:12075506, PubMed:15317753, PubMed:18611980). Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone (PubMed:10587585). Required for cardiac homeostasis (PubMed:10580070, PubMed:12927431, PubMed:18611980, PubMed:23666920).
Uniprot IDP02545
Protein Accession #NP_001244303
Nucleotide Accession #NM_001257374.2
Protein Size (# AA)664
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