LMF1 ELISA Kit (Human) (OKCD02694)

Catalog No: OKCD02694

Size:96WELLS

Human LMF1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase Maturation Factor 1 (LMF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase Maturation Factor 1 (LMF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase Maturation Factor 1 (LMF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase Maturation Factor 1 (LMF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase Maturation Factor 1 (LMF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase Maturation Factor 1 (LMF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LMF1 Microplate: 96 Wells (12 x 8 Well strips)
  • LMF1 Lyophilized Standard: 2
  • 100X Biotinylated LMF1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase Maturation Factor 1 (LMF1). No significant cross-reactivity or interference between Lipase Maturation Factor 1 (LMF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LMF1
• Gene Full Name: lipase maturation factor 1
• Alias Symbols: C16orf26;HMFN1876;JFP11;lipase maturation factor 1;TMEM112;TMEM112A;transmembrane protein 112.
• NCBI Gene Id: 64788
• Protein Name: Lipase maturation factor 1
• Description of Target: Involved in the maturation of specific proteins in the endoplasmic reticulum. Required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway. Each LMF1 molecule chaperones 50 or more molecules of LPL.
• Uniprot ID: Q96S06
• Protein Accession #: NP_073610.2
• Nucleotide Accession #: NM_022773.2
• Protein Size (# AA): 567