LIPG Antibody (OAEB01462)

Catalog No: OAEB01462 (Formerly GWB-EV0416)

Size:100UG

OAEB01462 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: LIPG
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 85%; Guinea Pig: 92%; Human: 100%; Mouse: 84%
• Peptide Sequence: C-RFNLRTSKDPEHEG
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al, J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.;
  PMID: 17356047). Recommended concentration: 0.3-1ug/ml.
  An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * Jin W, Wang X, Millar JS, Quertermous T, Rothblat GH, Glick JM, Rader DJ.; Hepatic proprotein convertases modulate HDL metabolism.; Cell Metab. 2007 Aug;6(2):129-36.; Pubmed 17681148

Target Info

• Gene Symbol: LIPG
• Gene Full Name: lipase G, endothelial type
• Alias Symbols: EDL;EL;endothelial cell-derived lipase;endothelial lipase;lipase, endothelial;lipoprotein lipase H;phospholipase A1;PRO719.
• NCBI Gene Id: 9388
• Protein Name: Endothelial lipase
• Uniprot ID: Q9Y5X9
• Protein Accession #: NP_006024.1
• Molecular Weight: 56.8