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Catalog No: OKEH00305
Size:96T
Price: $525.00
SKU
OKEH00305
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LIPF ELISA Kit (Human) : 96 Wells (OKEH00305)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LIPF ELISA Kit (Human) (OKEH00305) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIPF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for LIPF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIPF captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
LIPF Microplate96 Wells (12 x 8 Well strips)
LIPF Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated LIPF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Human Gastric triacylglycerol lipase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLIPF
Gene Full Namelipase F, gastric type
Alias Symbolsgastric lipase;gastric triacylglycerol lipase;GL;HGL;HLAL;lipase, gastric.
NCBI Gene Id8513
Protein NameP07098
Description of TargetGastric triacylglycerol lipase
Uniprot IDhttps://www.uniprot.org/uniprot/P07098
Protein Accession #NP_001185757.1
Nucleotide Accession #NM_001198828.1
Protein Size (# AA)398
Molecular Weight45 kDa
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