LIPC ELISA Kit (Mouse) (OKCD02691)

Catalog No: OKCD02691

Size:96WELLS

Mouse LIPC ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-104%	90-97%	97-104%	94-103%
  EDTA Plasma (n=5)	85-99%	89-101%	92-99%	82-98%
  Heparin Plasma (n=5)	93-105%	82-101%	97-105%	95-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic (LIPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hepatic (LIPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hepatic (LIPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase, Hepatic (LIPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-96	90
  EDTA Plasma (n=5)	95-104	101
  Heparin Plasma(n=5)	93-105	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LIPC Microplate: 96 Wells (12 x 8 Well strips)
  • LIPC Lyophilized Standard: 2
  • 100X Biotinylated LIPC Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma,other biological fluids
• Sensitivity: 0.126 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase, Hepatic (LIPC). No significant cross-reactivity or interference between Lipase, Hepatic (LIPC) and analogues was observed. Recovery
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lipc
• Gene Full Name: lipase, hepatic
• Alias Symbols: AI256194;H;hepatic lipase;hepatic triacylglycerol lipase;HL;Hpl;lipase member C;lysophospholipase;phospholipase A1.
• NCBI Gene Id: 15450
• Protein Name: Hepatic triacylglycerol lipase
• Description of Target: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
• Uniprot ID: P27656
• Protein Accession #: NP_001311401.1
• Nucleotide Accession #: NM_001324472.1
• Protein Size (# AA): 510