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Catalog No: OKCD00901 (Formerly GWB-KBBAY8)
Size:96WELLS
Price: $700.00
SKU
OKCD00901
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Datasheets/ManualsPrintable datasheet for LIPA ELISA Kit (Mouse) (OKCD00901)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
erum(n=5)93-101%79-93%96-104%87-105%
EDTA plasma(n=5)97-105%90-97%78-104%98-105%
heparin plasma(n=5)84-97%94-101%80-94%83-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10299
EDTA plasma(n=5)88-10394
heparin plasma(n=5)80-9386
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase A, Lysosomal Acid (LIPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase A, Lysosomal Acid (LIPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Lipa Microplate96 Wells (12 x 8 Well Strips)
Lipa Lyophilized Standard2 x 20 ng
100X Biotinylated Lipa Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.126 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase A, Lysosomal Acid (LIPA). No significant cross-reactivity or interference between Lipase A, Lysosomal Acid (LIPA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLipa
Gene Full Namelysosomal acid lipase A
Alias SymbolsAA960673;acid cholesteryl ester hydrolase;cholesteryl esterase;L;Lal;Li;Lip1;Lip-1;lipase A;lysosomal acid lipase 1;lysosomal acid lipase/cholesteryl ester hydrolase;sterol esterase.
NCBI Gene Id16889
Protein NameLysosomal acid lipase/cholesteryl ester hydrolase
Description of TargetCrucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation (By similarity).
Uniprot IDQ9Z0M5
Protein Accession #NP_001104570
Nucleotide Accession #NM_001111100.1
Protein Size (# AA)397
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