LIN28 Antibody - N-terminal region (ARP34477_P050)

Catalog No: ARP34477_P050

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WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate

Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-LIN28 antibody (ARP34477_P050)

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Pig, Rabbit, Sheep, Yeast
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LIN28
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 91%; Guinea Pig: 86%; Human: 100%; Mouse: 90%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Yeast: 77%
• Peptide Sequence: Synthetic peptide located within the following region: GSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRM
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-LIN28A (ARP34477_P050) antibody is Catalog # AAP34477 (Previous Catalog # AAPP23517)
• Reference: Gregory,S.G., (2006) Nature 441 (7091), 315-321

Target Info

• Gene Symbol: LIN28A
• Gene Full Name: Lin-28 homolog A (C. elegans)
• Alias Symbols: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
• NCBI Gene Id: 79727
• Protein Name: Protein lin-28 homolog A
• Description of Target: LIN28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. The association of LIN28 with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. IT binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. LIN28 is Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression.
• Uniprot ID: Q9H9Z2
• Protein Accession #: NP_078950
• Nucleotide Accession #: NM_024674
• Protein Size (# AA): 209
• Molecular Weight: 23kDa
• Protein Interactions: HNRNPUL2; HNRNPA3; DHX36; SREK1; TRIM71; LARP4; TGS1; DHX57; GADD45GIP1; LTV1; NOA1; DDX50; KRI1; NOL6; METTL17; NOC3L; ZC3HAV1; PPAN; CCBL2; MEPCE; TSR1; NHP2; NOP10; STRBP; BRIX1; RBM28; PHAX; SRRT; LARP7; DDX41; HP1BP3; NOB1; PRPF19; ZBTB11; SND1; AATF