Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02688
Size:96WELLS
Price: $725.00
SKU
OKCD02688
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for LIF ELISA kit (Bovine)
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor (LIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor (LIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor (LIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukemia Inhibitory Factor (LIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukemia Inhibitory Factor (LIF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukemia Inhibitory Factor (LIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LIF Microplate96 Wells (12 x 8 Well strips)
LIF Lyophilized Standard2
100X Biotinylated LIF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity12.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leukemia Inhibitory Factor (LIF). No significant cross-reactivity or interference between Leukemia Inhibitory Factor (LIF) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLIF
Gene Full NameLIF interleukin 6 family cytokine
Alias SymbolsbLIF;cholinergic differentiation factor;leukemia inhibitory factor;leukemia inhibitory factor (cholinergic differentiation factor).
NCBI Gene Id280840
Protein NameQ27956
Description of TargetLeukemia inhibitory factor
Uniprot IDhttps://www.uniprot.org/uniprot/Q27956
Protein Accession #NP_776356.1
Nucleotide Accession #NM_173931.1
Protein Size (# AA)202
Write Your Own Review
You're reviewing:LIF ELISA Kit (Bovine) (OKCD02688)
Your Rating
We found other products you might like!