LGMN ELISA Kit (Mouse) (OKCD02687)

Catalog No: OKCD02687

Size:96WELLS

Mouse LGMN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-95%	90-97%	99-105%	93-105%
  EDTA Plasma (n=5)	78-97%	85-92%	80-91%	83-92%
  Heparin Plasma (n=5)	83-102%	99-105%	84-95%	81-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Legumain (LGMN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Legumain (LGMN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Legumain (LGMN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Legumain (LGMN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-103	101
  EDTA Plasma (n=5)	79-95	86
  Heparin Plasma(n=5)	90-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Legumain (LGMN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Legumain (LGMN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LGMN Microplate: 96 Wells (12 x 8 Well strips)
  • LGMN Lyophilized Standard: 2
  • 100X Biotinylated LGMN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Legumain (LGMN). No significant cross-reactivity or interference between Legumain (LGMN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lgmn
• Gene Full Name: legumain
• Alias Symbols: A;AEP;AI746452;asparaginyl endopeptidase;AU022324;legumain;Pr;preprolegumain;Protease, cysteine 1;protease, cysteine, 1;Prsc1.
• NCBI Gene Id: 19141
• Protein Name: Legumain
• Description of Target: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.
• Uniprot ID: O89017
• Protein Accession #: NP_035305.1
• Nucleotide Accession #: NM_011175.3
• Protein Size (# AA): 435