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Catalog No: OKCD00594 (Formerly GWB-KBBJA6)
Size:96WELLS
Price: $675.00
SKU
OKCD00594
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Datasheets/ManualsPrintable datasheet for LGI3 ELISA Kit (Human) (OKCD00594)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-105%87-94%98-105%83-104%
EDTA Plasma (n=5)80-91%96-104%78-101%86-101%
Heparin Plasma (n=5)85-93%79-93%95-104%88-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat LGI Family, Member 3 (LGI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat LGI Family, Member 3 (LGI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat LGI Family, Member 3 (LGI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Rich Repeat LGI Family, Member 3 (LGI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9684
EDTA plasma(n=5)87-10197
heparin plasma(n=5)95-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat LGI Family, Member 3 (LGI3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat LGI Family, Member 3 (LGI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LGI3 Microplate96 Wells (12 x 8 Well Strips)
LGI3 Lyophilized Standard2 x 10 ng
100X Biotinylated LGI3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat LGI Family, Member 3 (LGI3). No significant cross-reactivity or interference between Leucine Rich Repeat LGI Family, Member 3 (LGI3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLGI3
Gene Full Nameleucine rich repeat LGI family member 3
Alias Symbolsleucine-rich glioma-inactivated protein 3;leucine-rich repeat LGI family member 3;LGI1-like protein 4;LGIL4.
NCBI Gene Id203190
Protein NameLeucine-rich repeat LGI family member 3
Description of TargetMay participate in the regulation of neuronal exocytosis.
Uniprot IDQ8N145
Protein Accession #NP_644807
Nucleotide Accession #NM_139278.2
Protein Size (# AA)548
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