LGALS3BP ELISA Kit (Mouse) (OKCD02686)

Catalog No: OKCD02686

Size:96WELLS

Mouse LGALS3BP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-101%	98-105%	88-97%	88-97%
  EDTA Plasma (n=5)	97-105%	94-102%	88-103%	97-105%
  Heparin Plasma (n=5)	98-105%	78-101%	88-101%	94-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	81-97	86
  Heparin Plasma(n=5)	84-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LGALS3BP Microplate: 96 Wells (12 x 8 Well strips)
  • LGALS3BP Lyophilized Standard: 2
  • 100X Biotinylated LGALS3BP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.069 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). No significant cross-reactivity or interference between Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lgals3bp
• Gene Full Name: lectin, galactoside-binding, soluble, 3 binding protein
• Alias Symbols: 90K;CyC;CyCAP;cyp-C-associated protein;galectin-3-binding protein;Lectin galactoside-binding soluble 3-binding protein;MAC-2;MAC-2BP;mama;P;peptidylprolyl isomerase C-associated protein;Ppicap;Protein MAMA;Tango;Tango10b.
• NCBI Gene Id: 19039
• Protein Name: Galectin-3-binding protein
• Description of Target: Promotes integrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells (By similarity).
• Uniprot ID: Q07797
• Protein Accession #: NP_035280.1
• Nucleotide Accession #: NM_011150.2
• Protein Size (# AA): 577