Catalog No: OKCD00741 (Formerly GWB-KBBTH4)
Size:96WELLS
Price: $650.00
SKU
OKCD00741
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for LGALS12 ELISA Kit (Human) (OKCD00741)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-99%78-92%97-105%91-102%
EDTA plasma(n=5)87-98%78-101%94-105%95-103%
heparin plasma(n=5)88-104%85-101%78-92%78-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 12 (GAL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 12 (GAL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 12 (GAL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 12 (GAL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-10193
EDTA plasma(n=5)84-9591
heparin plasma(n=5)83-9789
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 12 (GAL12) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 12 (GAL12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LGALS12 Microplate96 Wells (12 x 8 Well Strips)
LGALS12 Lyophilized Standard2 x 2 ng
100X Biotinylated LGALS12 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 12.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Galectin 12 (GAL12). No significant cross-reactivity or interference between Galectin 12 (GAL12) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolLGALS12
Gene Full Namegalectin 12
Alias SymbolsGAL12;galectin-12;galectin-related inhibitor of proliferation;GRIP1;lectin, galactoside-binding, soluble, 12;testicular secretory protein Li 26.
NCBI Gene Id85329
Protein NameGalectin-12
Description of TargetBinds lactose. May participate in the apoptosis of adipocytes.
Uniprot IDQ96DT0
Protein Accession #NP_001136007
Nucleotide Accession #NM_001142535.1
Protein Size (# AA)336
Write Your Own Review
You're reviewing:LGALS12 ELISA Kit (Human) (OKCD00741)
Your Rating