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Catalog No: OKEH03989
Size:96T
Price: $425.00
SKU
OKEH03989
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LGALS1 ELISA Kit (Human) (OKEH03989) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGALS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGALS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGALS1 captured in the well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 3.7% (n = 20)
Mean Inter-assay CV%: <= 7.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-LGALS1 Microplate96 Wells (12 x 8 Well Strips)
LGALS1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
LGALS1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.33 ng/mL
SpecificityNatural and recombinant Human Galectin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLGALS1
Gene Full Namegalectin 1
Alias Symbols14 kDa laminin-binding protein;14 kDa lectin;beta-galactoside-binding lectin L-14-I;beta-galactoside-binding protein 14kDa;epididymis secretory sperm binding protein;gal-1;GAL1;galaptin;galectin-1;GBP;HBL;HLBP14;HPL;lactose-binding lectin 1;Lectin galactoside-binding soluble 1;lectin, galactoside-binding, soluble, 1;putative MAPK-activating protein PM12;S-Lac lectin 1.
NCBI Gene Id3956
Protein NameP09382
Description of TargetGalectin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P09382
Protein Accession #NP_002296.1
Nucleotide Accession #NM_002305.3
Protein Size (# AA)135
Molecular Weight15 kDa
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