Catalog No: OKCA01337
Size:96 Wells
Price: $750.00
SKU
OKCA01337
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat10598-111
1:29990-104
1:49786-103
1:810395-107
ELISA Kit PrincipleAviva Systems Biology LEAP2 ELISA Kit (Human) (OKCA01337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEAP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEAP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LEAP2 captured in the well.
ELISA Kit Range0.312 - 20 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9991-104
EDTA Plasma (n=4)10295-107
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level OKCA01337 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01337 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
LEAP2 Microplate96 Wells (12 x 8 Well strips)
LEAP2 Lyophilized Standard2 x 20 ng
100X Biotinylated LEAP2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolLEAP2
Gene Full Nameliver enriched antimicrobial peptide 2
Alias SymbolsLEAP-2
NCBI Gene Id116842
Protein NameLiver-expressed antimicrobial peptide 2
Description of TargetHas an antimicrobial activity.
Uniprot IDQ969E1
Protein Accession #NP_443203.1
Nucleotide Accession #NM_052971.2
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