Catalog No: OKEH04081
Size:96T
Price: $425.00
SKU
OKEH04081
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Ldlr ELISA Kit (Mouse) (OKEH04081) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ldlr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ldlr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ldlr captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.5% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Ldlr Microplate96 Wells (12 x 8 Well Strips)
Ldlr Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Ldlr Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.083 ng/mL
SpecificityNatural and recombinant Mouse Low-density lipoprotein receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLdlr
Gene Full Namelow density lipoprotein receptor
Alias SymbolsHlb301;LDL receptor;low-density lipoprotein receptor.
NCBI Gene Id16835
Protein NameP35951
Description of TargetLow-density lipoprotein receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P35951
Protein Accession #NP_001239587.1
Nucleotide Accession #NM_001252658.1
Protein Size (# AA)862
Molecular Weight95 kDa
Write Your Own Review
You're reviewing:LDLR ELISA Kit (Mouse) (OKEH04081)
Your Rating
We found other products you might like!