Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01225
Size:96T
Price: $525.00
SKU
OKEH01225
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LCN12 ELISA Kit (Human) (OKEH01225) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCN12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCN12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCN12 captured in well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 10.4% (n = 20)
ELISA Kit Component
ComponentAmount
LCN12 Microplate96 Wells (12 x 8 Well strips)
LCN12 Lyophilized Standard2
100X Biotinylated LCN12 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Human Epididymal-specific lipocalin-12
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLCN12
Gene Full Namelipocalin 12
Alias Symbolsepididymal-specific lipocalin-12;epididymis secretory sperm binding protein;lipocalcin 12.
NCBI Gene Id286256
Protein NameQ6JVE5
Description of TargetEpididymal-specific lipocalin-12
Uniprot IDhttps://www.uniprot.org/uniprot/Q6JVE5
Protein Accession #NP_848631.2
Nucleotide Accession #NM_178536.3
Protein Size (# AA)192
Molecular Weight22 kDa
Write Your Own Review
You're reviewing:LCN12 ELISA Kit (Human) (OKEH01225)
Your Rating
We found other products you might like!