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Catalog No: OKEH01144
Size:96T
Price: $525.00
SKU
OKEH01144
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LCE3D ELISA Kit (Human) (OKEH01144) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCE3D has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCE3D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCE3D captured in well.
ELISA Kit Range0.625-40ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
LCE3D Microplate96 Wells (12 x 8 Well strips)
LCE3D Lyophilized Standard2
100X Biotinylated LCE3D Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.15 ng/mL
SpecificityNatural and recombinant Human Late cornified envelope protein 3D
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLCE3D
Gene Full Namelate cornified envelope 3D
Alias Symbolslate cornified envelope protein 3D;late envelope protein 16;LEP16;small proline rich-like (epidermal differentiation complex) 6A;small proline rich-like (epidermal differentiation complex) 6B;small proline-rich-like epidermal differentiation complex protein 6A;small proline-rich-like epidermal differentiation complex protein 6B;SPRL6A;SPRL6B.
NCBI Gene Id84648
Protein NameQ9BYE3
Description of TargetLate cornified envelope protein 3D
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BYE3
Protein Accession #NP_115952.1
Nucleotide Accession #NM_032563.1
Protein Size (# AA)92
Molecular Weight9 kDa
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