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Catalog No: OKCD02680
Size:96WELLS
Price: $700.00
SKU
OKCD02680
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Datasheets/ManualsPrintable datasheet for LCAT ELISA Kit (Mouse) (OKCD02680)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%82-95%78-97%95-105%
EDTA Plasma (n=5)86-94%97-105%80-102%79-93%
Heparin Plasma (n=5)86-96%80-93%80-90%96-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lecithin Cholesterol Acyltransferase (LCAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lecithin Cholesterol Acyltransferase (LCAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9085
EDTA Plasma (n=5)93-10196
Heparin Plasma(n=5)94-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-LCAT Microplate96 Wells (12 x 8 Well strips)
LCAT Lyophilized Standard2
100X Biotinylated LCAT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolLcat
Gene Full Namelecithin cholesterol acyltransferase
Alias Symbols1-alkyl-2-acetylglycerophosphocholine esterase;AI046659;D8Wsu61;D8Wsu61e;Lecithin-cholesterol acyltransferase;lecithin-cholesterol acyltransferase Lcat;PAF acetylhydrolase;phosphatidylcholine-sterol acyltransferase;phospholipid-cholesterol acyltransferase;platelet-activating factor acetylhydrolase.
NCBI Gene Id16816
Protein NamePhosphatidylcholine-sterol acyltransferase
Description of TargetCentral enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:19065001). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed:19065001). Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed:19065001). Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:19065001). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107).
Uniprot IDP16301
Protein Accession #NP_032516.2
Nucleotide Accession #NM_008490.2
Protein Size (# AA)438
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