LCAT ELISA Kit (Human) (OKEH04779)

Catalog No: OKEH04779

Size:96T

Human LCAT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology LCAT ELISA Kit (Human) (OKEH04779) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCAT captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • LCAT Microplate: 96 Wells (12 x 8 Well Strips)
  • LCAT Standard: 2
  • Sample Diluent: 1 x 20 mL
  • LCAT Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.098 ng/mL
• Specificity: Natural and recombinant Human Phosphatidylcholine-sterol acyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LCAT
• Gene Full Name: lecithin-cholesterol acyltransferase
• Alias Symbols: 1-alkyl-2-acetylglycerophosphocholine esterase;Lecithin-cholesterol acyltransferase;PAF acetylhydrolase;phosphatidylcholine-sterol acyltransferase;phosphatidylcholine--sterol O-acyltransferase;phospholipid-cholesterol acyltransferase;platelet-activating factor acetylhydrolase;testicular secretory protein Li 24.
• NCBI Gene Id: 3931
• Protein Name: P04180
• Description of Target: Phosphatidylcholine-sterol acyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/P04180
• Protein Accession #: NP_000220.1
• Nucleotide Accession #: NM_000229.1
• Protein Size (# AA): 440
• Molecular Weight: 50 kDa