LARS2 Antibody (OAGA00769)

Catalog No: OAGA00769

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
OAGA00769 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using LARS2(OAGA00769) antibody at 1:500 dilution.

LARS2 antibody [N1N2], N-term detects LARS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: LARS2 protein stained by LARS2 antibody [N1N2], N-term (OAGA00769) diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq]
  Positive Controls: 293T, H1299, HeLaS3, HepG2, Raji
  Cellular Localization: Mitochondrion matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 204 of LARS2 (Uniprot ID#Q15031)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: LARS2
• Gene Full Name: Probable leucine--tRNA ligase, mitochondrial
• Alias Symbols: HLASA, LEURS, PRLTS4, mtLeuRS
• NCBI Gene Id: 23395
• Protein Name: Probable leucine--tRNA ligase, mitochondrial
• Uniprot ID: Q15031
• Protein Accession #: NP_056155.1
• Molecular Weight: 102 kDa