Catalog No: OKEH00192
Size:96T
Price: $525.00
SKU
OKEH00192
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LAPTM4B ELISA Kit (Human) : 96 Wells (OKEH00192)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LAPTM4B ELISA Kit (Human) (OKEH00192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAPTM4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAPTM4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAPTM4B captured in well.
ELISA Kit Range0.47-30ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
LAPTM4B Microplate96 Wells (12 x 8 Well strips)
LAPTM4B Lyophilized Standard2
100X Biotinylated LAPTM4B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.21 ng/mL
SpecificityNatural and recombinant Human Lysosomal-associated transmembrane protein 4B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLAPTM4B
Gene Full Namelysosomal protein transmembrane 4 beta
Alias SymbolsLAPTM4beta;LC27;lysosomal associated protein transmembrane 4 beta;lysosomal-associated transmembrane protein 4B;lysosome-associated transmembrane protein 4-beta.
NCBI Gene Id55353
Protein NameQ86VI4
Description of TargetLysosomal-associated transmembrane protein 4B
Uniprot IDhttps://www.uniprot.org/uniprot/Q86VI4
Protein Accession #NP_060877
Nucleotide Accession #NM_018407
Protein Size (# AA)370
Molecular Weight41 kDa
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