LAMP1 ELISA Kit (Mouse) (OKCA01715)

Catalog No: OKCA01715

Size:96 Wells

Mouse LAMP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	105	99-109
  1:2	86	81-89
  1:4	92	87-98
  1:8	89	85-93

• ELISA Kit Principle: Aviva Systems Biology Lamp1 ELISA Kit (Mouse) (OKCA01715) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lamp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lamp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Lamp1 captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	97	91-102
  EDTA Plasma (n=4)	103	98-107

• ELISA Kit Component:
  • Lamp1 Microplate: 96 Wells (12 x 8 Well strips)
  • Lamp1 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated Lamp1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: LAMP1
• Gene Full Name: lysosomal-associated membrane protein 1
• Alias Symbols: Pe, Lam, P2B, Perk, CD107, LGP-A, CD107a, Lamp-1, LGP-120
• NCBI Gene Id: 16783
• Protein Name: Lysosome-associated membrane glycoprotein 1
• Description of Target: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
• Uniprot ID: P11438
• Protein Accession #: NP_001304282.1
• Nucleotide Accession #: NM_001317353.1