Catalog No: OKDD00368 (Formerly GWB-KBBHG8)
Size:96 Wells
Price: $750.00
SKU
OKDD00368
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Datasheets/ManualsPrintable datasheet for LAMP1 ELISA Kit (Human) (OKDD00368)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology LAMP1 ELISA Kit (Human) (OKDD00368) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LAMP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMP1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LAMP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LAMP1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LAMP1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-LAMP1 Microplate96 Wells (12 x 8 Well Strips)
LAMP1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X LAMP1 Biotinylated Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Detection Conjugate1 x 120 ÂuL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates or other biological fluids
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of LAMP1. No significant cross-reactivity or interference between LAMP1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolLAMP1
Gene Full Namelysosomal associated membrane protein 1
Alias SymbolsCD107 antigen-like family member A;CD107a;LAMP-1;LAMPA;LGP120;lysosome-associated membrane glycoprotein 1;lysosome-associated membrane protein 1.
NCBI Gene Id3916
Protein NameLysosome-associated membrane glycoprotein 1
Description of TargetPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Uniprot IDP11279
Protein Accession #NP_005552.3
Nucleotide Accession #NM_005561.3
Protein Size (# AA)417
Molecular Weight45 kDa
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