LAMC2 ELISA Kit (Human) (OKEH04464)

Catalog No: OKEH04464

Size:96T

Human LAMC2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology LAMC2 ELISA Kit (Human) (OKEH04464) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMC2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for LAMC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMC2 captured in the well.
• ELISA Kit Range: 62.5-4000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.7% (n = 20)
  Mean Inter-assay CV%: <= 8.4% (n = 20)
• ELISA Kit Component:
  • Anti-LAMC2 Microplate: 96 Wells (12 x 8 Well Strips)
  • LAMC2 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • LAMC2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 21 pg/mL
• Specificity: Natural and recombinant Human Laminin subunit gamma-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LAMC2
• Gene Full Name: laminin subunit gamma 2
• Alias Symbols: B2T;BM600;BM600-100kDa;cell-scattering factor 140 kDa subunit;CSF;CSF 140 kDa subunit;EBR2;EBR2A;epiligrin subunit gamma;kalinin subunit gamma;Kalinin/nicein/epiligrin 100 kDa subunit;ladsin 140 kDa subunit;LAMB2T;laminin B2t chain;laminin subunit gamma-2;laminin, gamma 2;Laminin-5 subunit gamma;LAMNB2;large adhesive scatter factor 140 kDa subunit;nicein subunit gamma.
• NCBI Gene Id: 3918
• Protein Name: Q13753
• Description of Target: Laminin subunit gamma-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q13753
• Protein Accession #: NP_005553.2
• Nucleotide Accession #: NM_005562.2
• Protein Size (# AA): 1193
• Molecular Weight: 131 KDa