LAMC1 ELISA Kit (Human) (OKCD02679)

Catalog No: OKCD02679

Size:96WELLS

Human LAMC1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	93-101%	83-104%	98-105%
  EDTA Plasma (n=5)	80-90%	89-97%	78-97%	90-104%
  Heparin Plasma (n=5)	93-101%	80-104%	85-102%	79-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Gamma 1 (LAMC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Gamma 1 (LAMC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Gamma 1 (LAMC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Laminin Gamma 1 (LAMC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-94	90
  EDTA Plasma (n=5)	81-103	92
  Heparin Plasma(n=5)	92-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Gamma 1 (LAMC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Gamma 1 (LAMC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-LAMC1 Microplate: 96 Wells (12 x 8 Well strips)
  • LAMC1 Lyophilized Standard: 2
  • 100X Biotinylated LAMC1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
• Sensitivity: < 1.13 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Laminin Gamma 1 (LAMC1). No significant cross-reactivity or interference between Laminin Gamma 1 (LAMC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LAMC1
• Gene Full Name: laminin subunit gamma 1
• Alias Symbols: LAMB2;laminin B2 chain;laminin subunit gamma-1;laminin, gamma 1 (formerly LAMB2);Laminin-1 subunit gamma;laminin-10 subunit gamma;laminin-11 subunit gamma;laminin-2 subunit gamma;laminin-3 subunit gamma;laminin-4 subunit gamma;laminin-6 subunit gamma;laminin-7 subunit gamma;laminin-8 subunit gamma;laminin-9 subunit gamma;S-LAM gamma;S-laminin subunit gamma.
• NCBI Gene Id: 3915
• Protein Name: Laminin subunit gamma-1
• Description of Target: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
• Uniprot ID: P11047
• Protein Accession #: NP_002284.3
• Nucleotide Accession #: NM_002293.3
• Protein Size (# AA): 1609