Catalog No: OKEH06335
Size:96T
Price: $625.00
SKU
OKEH06335
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology LALBA ELISA Kit (Pig) (OKEH06335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LALBA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LALBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LALBA captured in the well.
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 5.6% (n = 20)
Mean Inter-Assay Precision - <= 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
SpecificityNatural and recombinant Pig Alpha-lactalbumin
Assay InfoAssay Methodology: Lot dependent. Can either be Quantitative or Sandwich ELISA.
Gene SymbolLALBA
Gene Full Namelactalbumin alpha
Alias Symbolsalpha-lactalbumin;lactose synthase B protein.
NCBI Gene Id397647
Protein NameP18137
Description of TargetAlpha-lactalbumin
Uniprot IDhttps://www.uniprot.org/uniprot/P18137
Protein Accession #NP_001020385
Nucleotide Accession #NM_001025214
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