Catalog No: OKEH03892
Size:96T
Price: $625.00
SKU
OKEH03892
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03892
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LALBA ELISA Kit (Bovine) (OKEH03892) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LALBA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LALBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LALBA captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityMean Intra-Assay Precision - ≤ 5.1%, n = 20
Mean Inter-Assay Precision - ≤ 7.5%, n = 20
ELISA Kit Component
ComponentAmount
Anti-LALBA Microplate96 Wells (12 x 8 Well strips)
LALBA Lyophilized Standard2
100X Biotinylated LALBA Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.56 ng/mL
SpecificityNatural and recombinant Bovine Alpha-lactalbumin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLALBA
Gene Full Namelactalbumin alpha
Alias Symbolsa-LACTA;alfaLA;alpha lactabumin;alpha lactalbumin;alpha-lactalbumin;alpha-lactalbumin A;alpha-lactalbumin B;alpha-lactalbumin protein variant D;lactose synthase B protein.
NCBI Gene Id281894
Protein NameP00711
Description of TargetAlpha-lactalbumin
Uniprot IDhttps://www.uniprot.org/uniprot/P00711
Protein Accession #NP_776803.1
Nucleotide Accession #NM_174378.2
Protein Size (# AA)142
Molecular Weight16 kDa
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