KRT81 ELISA Kit (Human) (OKCD00635)

Catalog No: OKCD00635 (Formerly GWB-KBBZM6)

Size:96WELLS

Human KRT81 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 81 (KRT81). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 81 (KRT81). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 81 (KRT81), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Keratin 81 (KRT81) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 81 (KRT81) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRT81 Microplate: 96 Wells (12 x 8 Well Strips)
  • KRT81 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated KRT81 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 81 (KRT81). No significant cross-reactivity or interference between Keratin 81 (KRT81) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KRT81
• Gene Full Name: keratin 81
• Alias Symbols: ghHkb1;hair keratin K2.9;hard keratin, type II, 1;Hb-1;HB1;hHAKB2-1;K81;keratin 81, type II;keratin, hair, basic, 1;keratin, type II cuticular Hb1;Keratin-81;KRTHB1;metastatic lymph node 137 gene protein;MLN137;type II hair keratin Hb1;type-II keratin Kb21.
• NCBI Gene Id: 3887
• Protein Name: Keratin, type II cuticular Hb1
• Description of Target: Keratin, type II cuticular Hb1
• Uniprot ID: Q14533
• Protein Accession #: NP_002272
• Nucleotide Accession #: NM_002281.3
• Protein Size (# AA): 505