Catalog No: OKEH04670
Size:96T
Price: $525.00
SKU
OKEH04670
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology KRT20 ELISA Kit (Human) (OKEH04670) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KRT20 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for KRT20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KRT20 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-KRT20 Microplate96 Wells (12 x 8 Well Strips)
KRT20 Lyophilized Standard2
Sample Diluent1 x 20 mL
KRT20 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Keratin, type I cytoskeletal 20
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKRT20
Gene Full Namekeratin 20
Alias SymbolsCD20;CK20;CK-20;cytokeratin 20;Cytokeratin-20;K20;keratin 20, type I;keratin, type I cytoskeletal 20;Keratin-20;KRT21;protein IT.
NCBI Gene Id54474
Protein NameP35900
Description of TargetKeratin, type I cytoskeletal 20
Uniprot IDhttps://www.uniprot.org/uniprot/P35900
Protein Accession #NP_061883.1
Nucleotide Accession #NM_019010.2
Protein Size (# AA)424
Molecular Weight48 KDa
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