KRT13 ELISA Kit (Human) (OKCD02675)

Catalog No: OKCD02675

Size:96WELLS

Human KRT13 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-99%	86-99%	94-101%	80-101%
  EDTA Plasma (n=5)	80-95%	85-105%	92-105%	93-102%
  Heparin Plasma (n=5)	78-90%	80-104%	89-102%	92-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 13 (CK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 13 (CK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 13 (CK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 13 (CK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-99	93
  EDTA Plasma (n=5)	78-89	84
  Heparin Plasma(n=5)	90-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 13 (CK13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 13 (CK13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRT13 Microplate: 96 Wells (12 x 8 Well strips)
  • KRT13 Lyophilized Standard: 2
  • 100X Biotinylated KRT13 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 6.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 13 (KRT13). No significant cross-reactivity or interference between Keratin 13 (KRT13) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KRT13
• Gene Full Name: keratin 13
• Alias Symbols: CK13;CK-13;cytokeratin 13;Cytokeratin-13;K13;keratin 13, type I;keratin, type I cytoskeletal 13;Keratin-13;WSN2.
• NCBI Gene Id: 3860
• Protein Name: Keratin, type I cytoskeletal 13
• Description of Target: Keratin, type I cytoskeletal 13
• Uniprot ID: P13646
• Protein Accession #: NP_002265.2
• Nucleotide Accession #: NM_002274.3
• Protein Size (# AA): 458