Catalog No: OKEH06680
Size:96T
Price: $625.00
SKU
OKEH06680
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Sbspon ELISA Kit (Bovine) (OKEH06680) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sbspon has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sbspon is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sbspon captured in the well.
ELISA Kit Range0.78-50ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Sbspon Microplate96 Wells (12 x 8 Well strips)
Sbspon Lyophilized Standard2 x 50 ng
100X Sbspon HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.32 ng/mL
SpecificityNatural and recombinant Bovine RPE-spondin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSBSPON
Gene Full Namesomatomedin B and thrombospondin type 1 domain containing
Alias SymbolsC14H8orf84;RPESP;RPE-spondin;somatomedin-B and thrombospondin type-1 domain-containing protein.
NCBI Gene Id525332
Protein NameQ32L50
Description of TargetSomatomedin-B and thrombospondin type-1 domain-containing protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q32L50
Protein Accession #NP_001069337
Nucleotide Accession #NM_001075869
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