KPNA1 Antibody (OAPB01179)

Catalog No: OAPB01179

Size:100UG

Western blot analysis of KPNA1 in Hela cell lysate with KPNA1 antibody at 1ug/mL.

Immunofluorescence of KPNA1 in K562 cells with KPNA1 antibody at 20 ug/mL.

Immunocytochemistry of KPNA1 in HeLa cells with KPNA1 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. KPNA1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: KPNA1 antibody can be stored at 4C for three months and-20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: KPNA1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA1. The immunogen is located within amino acids 30 - 80 of KPNA1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-KPNA1 antibody is Catalog # OEPB03295
• Application Info: KPNA1 antibody can be used for detection of KPNA1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.
• Reference: Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.;
  Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49. ;
  Simkus C, Makiya M and Jones JM. Karyopherin alpha 1 is a putative substrate of the RAG1 ubiquitin ligase. Mol. Immunol.2009; 46:1319-25.;
  Reid SP, Valmas C, and Martinez O. Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1. J. Virol.2007; 81:13469-77.

Target Info

• Gene Symbol: KPNA1
• Gene Full Name: karyopherin subunit alpha 1
• Alias Symbols: importin alpha 5;importin subunit alpha-1;importin subunit alpha-5;importin-alpha-S1;IPOA5;karyopherin alpha 1 (importin alpha 5);Karyopherin subunit alpha-1;NPI-1;nucleoprotein interactor 1;RAG cohort protein 2;RCH2;recombination activating gene cohort 2;SRP1;SRP1-beta.
• NCBI Gene Id: 3836
• Protein Name: Importin subunit alpha-5|importin subunit alpha-5 [Homo sapiens]
• Description of Target: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
• Uniprot ID: P52294
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_002255