Catalog No: OKEH00566
Size:96T
Price: $625.00
SKU
OKEH00566
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

KNG1 ELISA Kit (Bovine) : 96 Wells (OKEH00566)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KNG1 ELISA Kit (Bovine) (OKEH00566) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine KNG1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KNG1 and incubated. The KNG1 found in the sample or standards competes with the biotinylated KNG1 for limited binding sites on the immobilized anti-Bovine KNG1 antibody. Excess unbound biotinylated KNG1 and sample or standard KNG1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KNG1 captured in the well and inversely proportional to the amount of KNG1 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 6.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-KNG1 Microplate96 Wells (12 x 8 Well strips)
KNG1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X KNG1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.406 ng/mL
SpecificityNatural and recombinant Bovine Kininogen-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKNG1
Gene Full Namekininogen 1
Alias Symbolsbradykinin;kininogen I;kininogen II;kininogen-2;KNG;KNG2;thiol proteinase inhibitor.
NCBI Gene Id280833
Protein NameP01044
Description of TargetKininogen-1
Uniprot IDhttps://www.uniprot.org/uniprot/P01044
Protein Accession #NP_786968.2
Nucleotide Accession #NM_175774.3
Protein Size (# AA)621
Molecular Weight69 kDa
Write Your Own Review
You're reviewing:KNG1 ELISA Kit (Bovine) : 96 Wells (OKEH00566)
Your Rating
We found other products you might like!